7MC3

Solution structure of Miz-1 zinc finger 12

7MC3 の概要

• エントリーDOI: 10.2210/pdb7mc3/pdb
• NMR情報: BMRB: 50842
• 分子名称: Isoform 2 of Zinc finger and BTB domain-containing protein 17, ZINC ION (2 entities in total)
• 機能のキーワード: zinc finger, c2h2, dna binding, transcription factor, dna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9850.77

構造登録者

Boisvert, O.,Lavigne, P. (登録日: 2021-04-01, 公開日: 2021-04-21, 最終更新日: 2024-05-15)

主引用文献

Boisvert, O.,Letourneau, D.,Delattre, P.,Tremblay, C.,Jolibois, E.,Montagne, M.,Lavigne, P.
Zinc Fingers 10 and 11 of Miz-1 undergo conformational exchange to achieve specific DNA binding.
Structure, 30:623-636.e5, 2022

PubMed Abstract: Miz-1 (ZBTB17) is a poly-zinc finger BTB/POZ transcription factor with 12 consecutive C2H2 zinc fingers (ZFs) that binds transcriptional start sites (TSSs) to regulate the expression of genes involved in cell development and proliferation. As of now, it is not known which of the 12 consecutive ZFs are responsible for the recognition of the 24 base pair consensus sequence found at these TSSs. Evidence suggests ZFs 7-12 plays this role. We provide validation for this and describe the structural and dynamical characterization of unprecedented conformational exchange in the linker between ZFs 10 and 11. This conformational exchange uncouples ZFs 7-10 from 11 and 12 and promotes a scanning-recognition mechanism through which the two segments cooperate to bind two sub-sites at both ends of the consensus. We further show that this can result in the coiling of TSSs as part of Miz-1's mechanism of transcriptional transactivation.

PubMed: 34963061
DOI: 10.1016/j.str.2021.12.001

実験手法

SOLUTION NMR