7MC2

Solution structure of Miz-1 Zinc finger 11 H586Y

Summary for 7MC2

• Entry DOI: 10.2210/pdb7mc2/pdb
• NMR Information: BMRB: 50842
• Descriptor: Isoform 2 of Zinc finger and BTB domain-containing protein 17, ZINC ION (2 entities in total)
• Functional Keywords: zinc finger, c2h2, dna binding, transcription factor, dna binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 9850.77

Authors

Boisvert, O.,Lavigne, P. (deposition date: 2021-04-01, release date: 2021-04-28, Last modification date: 2024-05-15)

Primary citation

Boisvert, O.,Letourneau, D.,Delattre, P.,Tremblay, C.,Jolibois, E.,Montagne, M.,Lavigne, P.
Zinc Fingers 10 and 11 of Miz-1 undergo conformational exchange to achieve specific DNA binding.
Structure, 30:623-636.e5, 2022

PubMed Abstract: Miz-1 (ZBTB17) is a poly-zinc finger BTB/POZ transcription factor with 12 consecutive C2H2 zinc fingers (ZFs) that binds transcriptional start sites (TSSs) to regulate the expression of genes involved in cell development and proliferation. As of now, it is not known which of the 12 consecutive ZFs are responsible for the recognition of the 24 base pair consensus sequence found at these TSSs. Evidence suggests ZFs 7-12 plays this role. We provide validation for this and describe the structural and dynamical characterization of unprecedented conformational exchange in the linker between ZFs 10 and 11. This conformational exchange uncouples ZFs 7-10 from 11 and 12 and promotes a scanning-recognition mechanism through which the two segments cooperate to bind two sub-sites at both ends of the consensus. We further show that this can result in the coiling of TSSs as part of Miz-1's mechanism of transcriptional transactivation.

PubMed: 34963061
DOI: 10.1016/j.str.2021.12.001

Experimental method

SOLUTION NMR