7M78

Room Temperature XFEL Crystallography reveals asymmetry in the vicinity of the two phylloquinones in Photosystem I

7M78 の概要

• エントリーDOI: 10.2210/pdb7m78/pdb
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit XI, Photosystem I reaction center subunit XII, ... (23 entities in total)
• 機能のキーワード: membrane complex, electron transport, photosynthesis
• 由来する生物種: Thermosynechococcus elongatus (strain BP-1); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 364412.80

構造登録者

Keable, S.M.,Simon, P.S.,Kolsch, A.,Kern, J.,Yachandra, V.K.,Zouni, A.,Yano, J. (登録日: 2021-03-26, 公開日: 2021-11-24, 最終更新日: 2024-12-25)

主引用文献

Keable, S.M.,Kolsch, A.,Simon, P.S.,Dasgupta, M.,Chatterjee, R.,Subramanian, S.K.,Hussein, R.,Ibrahim, M.,Kim, I.S.,Bogacz, I.,Makita, H.,Pham, C.C.,Fuller, F.D.,Gul, S.,Paley, D.,Lassalle, L.,Sutherlin, K.D.,Bhowmick, A.,Moriarty, N.W.,Young, I.D.,Blaschke, J.P.,de Lichtenberg, C.,Chernev, P.,Cheah, M.H.,Park, S.,Park, G.,Kim, J.,Lee, S.J.,Park, J.,Tono, K.,Owada, S.,Hunter, M.S.,Batyuk, A.,Oggenfuss, R.,Sander, M.,Zerdane, S.,Ozerov, D.,Nass, K.,Lemke, H.,Mankowsky, R.,Brewster, A.S.,Messinger, J.,Sauter, N.K.,Yachandra, V.K.,Yano, J.,Zouni, A.,Kern, J.
Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I.
Sci Rep, 11:21787-21787, 2021

PubMed Abstract: Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We have determined the structure of cyanobacterial PS I at room temperature (RT) using femtosecond X-ray pulses from an X-ray free electron laser (XFEL) that shows a clear expansion of the entire protein complex in the direction of the membrane plane, when compared to previous cryogenic structures. This trend was observed by complementary datasets taken at multiple XFEL beamlines. In the RT structure of PS I, we also observe conformational differences between the two branches in the reaction center around the secondary electron acceptors A and A. The π-stacked Phe residues are rotated with a more parallel orientation in the A-branch and an almost perpendicular confirmation in the B-branch, and the symmetry breaking PsaB-Trp673 is tilted and further away from A. These changes increase the asymmetry between the branches and may provide insights into the preferential directionality of electron transfer.

PubMed: 34750381
DOI: 10.1038/s41598-021-00236-3

実験手法

X-RAY DIFFRACTION (3 Å)