7M6A

High resolution structure of the membrane embedded skeletal muscle ryanodine receptor

これはPDB形式変換不可エントリーです。

7M6A の概要

• エントリーDOI: 10.2210/pdb7m6a/pdb
• EMDBエントリー: 23692
• 分子名称: Peptidyl-prolyl cis-trans isomerase FKBP1B, Ryanodine receptor 1, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: ryanodine receptor, calcium, membrane, liposome, membrane protein-isomerase complex, membrane protein/isomerase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 2314055.94

構造登録者

Melville, Z.,Kim, K.,Clarke, O.B.,Marks, A.R. (登録日: 2021-03-25, 公開日: 2021-09-08, 最終更新日: 2022-01-19)

主引用文献

Melville, Z.,Kim, K.,Clarke, O.B.,Marks, A.R.
High-resolution structure of the membrane-embedded skeletal muscle ryanodine receptor.
Structure, 30:172-, 2022

PubMed Abstract: The type 1 ryanodine receptor (RyR)/calcium release channel on the sarcoplasmic reticulum (SR) is required for skeletal muscle excitation-contraction coupling and is the largest known ion channel, composed of four 565-kDa protomers. Cryogenic electron microscopy (cryo-EM) studies of the RyR have primarily used detergent to solubilize the channel; in the present study, we have used cryo-EM to solve high-resolution structures of the channel in liposomes using a gel-filtration approach with on-column detergent removal to form liposomes and incorporate the channel simultaneously. This allowed us to resolve the structure of the channel in the primed and open states at 3.4 and 4.0 Å, respectively, with a single dataset. This method offers validation for detergent-based structures of the RyR and offers a starting point for utilizing a chemical gradient mimicking the SR, where Ca concentrations are millimolar in the lumen and nanomolar in the cytosol.

PubMed: 34469755
DOI: 10.1016/j.str.2021.08.001

実験手法

ELECTRON MICROSCOPY (3.36 Å)