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7M5C

Crystal Structure of human BAK in complex with WT BAK BH3 peptide

7M5C の概要
エントリーDOI10.2210/pdb7m5c/pdb
分子名称Bcl-2 homologous antagonist/killer, COPPER (II) ION, SULFATE ION, ... (4 entities in total)
機能のキーワードprotein-peptide complex, apoptosis
由来する生物種Homo sapiens (Human)
詳細
タンパク質・核酸の鎖数20
化学式量合計215039.63
構造登録者
Singh, G.,Aggarwal, A.,Moldoveanu, T. (登録日: 2021-03-23, 公開日: 2022-01-12, 最終更新日: 2024-10-09)
主引用文献Singh, G.,Guibao, C.D.,Seetharaman, J.,Aggarwal, A.,Grace, C.R.,McNamara, D.E.,Vaithiyalingam, S.,Waddell, M.B.,Moldoveanu, T.
Structural basis of BAK activation in mitochondrial apoptosis initiation.
Nat Commun, 13:250-250, 2022
Cited by
PubMed Abstract: BCL-2 proteins regulate mitochondrial poration in apoptosis initiation. How the pore-forming BCL-2 Effector BAK is activated remains incompletely understood mechanistically. Here we investigate autoactivation and direct activation by BH3-only proteins, which cooperate to lower BAK threshold in membrane poration and apoptosis initiation. We define in trans BAK autoactivation as the asymmetric "BH3-in-groove" triggering of dormant BAK by active BAK. BAK autoactivation is mechanistically similar to direct activation. The structure of autoactivated BAK BH3-BAK complex reveals the conformational changes leading to helix α1 destabilization, which is a hallmark of BAK activation. Helix α1 is destabilized and restabilized in structures of BAK engaged by rationally designed, high-affinity activating and inactivating BID-like BH3 ligands, respectively. Altogether our data support the long-standing hit-and-run mechanism of BAK activation by transient binding of BH3-only proteins, demonstrating that BH3-induced structural changes are more important in BAK activation than BH3 ligand affinity.
PubMed: 35017502
DOI: 10.1038/s41467-021-27851-y
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.06 Å)
構造検証レポート
Validation report summary of 7m5c
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-10-22に公開中

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