7M3U

Solution NMR Structure of PawS-Derived Peptide PDP-24

7M3U の概要

• エントリーDOI: 10.2210/pdb7m3u/pdb
• NMR情報: BMRB: 30889
• 分子名称: PawS-Derived Peptide PDP-24 (1 entity in total)
• 機能のキーワード: cyclic, aep-processed, bi-disulfide, plant protein
• 由来する生物種: Zinnia haageana
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3038.42

構造登録者

Payne, C.D.,Rosengren, K.J. (登録日: 2021-03-19, 公開日: 2021-03-31, 最終更新日: 2022-04-13)

主引用文献

Payne, C.D.,Vadlamani, G.,Hajiaghaalipour, F.,Muhammad, T.,Fisher, M.F.,Andersson, H.S.,Goransson, U.,Clark, R.J.,Bond, C.S.,Mylne, J.S.,Rosengren, K.J.
Solution NMR and racemic crystallography provide insights into a novel structural class of cyclic plant peptides.
Rsc Chem Biol, 2:1682-1691, 2021

PubMed Abstract: Head-to-tail cyclic and disulfide-rich peptides are natural products with applications in drug design. Among these are the PawS-Derived Peptides (PDPs) produced in seeds of the daisy plant family. PDP-23 is a unique member of this class in that it is twice the typical size and adopts two β-hairpins separated by a hinge region. The β-hairpins, both stabilised by a single disulfide bond, fold together into a V-shaped tertiary structure creating a hydrophobic core. In water two PDP-23 molecules merge their hydrophobic cores to form a square prism quaternary structure. Here, we synthesised PDP-23 and its enantiomer comprising d-amino acids and achiral glycine, which allowed us to confirm these solution NMR structural data by racemic crystallography. Furthermore, we discovered the related PDP-24. NMR analysis showed that PDP-24 does not form a dimeric structure and it has poor water solubility, but in less polar solvents adopts near identical secondary and tertiary structure to PDP-23. The natural role of these peptides in plants remains enigmatic, as we did not observe any antimicrobial or insecticidal activity. However, the plasticity of these larger PDPs and their ability to change structure under different conditions make them appealing peptide drug scaffolds.

PubMed: 34977583
DOI: 10.1039/d1cb00155h

実験手法

SOLUTION NMR