7M3N

Canine parvovirus and Fab14 asymmetric reconstruction

7M3N の概要

• エントリーDOI: 10.2210/pdb7m3n/pdb
• EMDBエントリー: 23658
• 分子名称: CPV Fab14 light chain, CPV Fab14 heavy chain, Capsid protein 2 (3 entities in total)
• 機能のキーワード: canine parvovirus, cpv, fab14, virus, virus-immune system complex, virus/immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 89791.45

構造登録者

Goteschius, D.J.,Hartmann, S.R.,Hafenstein, S.L. (登録日: 2021-03-18, 公開日: 2021-07-28, 最終更新日: 2025-05-28)

主引用文献

Goetschius, D.J.,Hartmann, S.R.,Organtini, L.J.,Callaway, H.,Huang, K.,Bator, C.M.,Ashley, R.E.,Makhov, A.M.,Conway, J.F.,Parrish, C.R.,Hafenstein, S.L.
High-resolution asymmetric structure of a Fab-virus complex reveals overlap with the receptor binding site.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Canine parvovirus is an important pathogen causing severe diseases in dogs, including acute hemorrhagic enteritis, myocarditis, and cerebellar disease. Overlap on the surface of parvovirus capsids between the antigenic epitope and the receptor binding site has contributed to cross-species transmission, giving rise to closely related variants. It has been shown that Mab 14 strongly binds and neutralizes canine but not feline parvovirus, suggesting this antigenic site also controls species-specific receptor binding. To visualize the conformational epitope at high resolution, we solved the cryogenic electron microscopy (cryo-EM) structure of the Fab-virus complex. We also created custom software, Icosahedral Subparticle Extraction and Correlated Classification, to solve a Fab-virus complex with only a few Fab bound per capsid and visualize local structures of the Fab-bound and -unbound antigenic sites extracted from the same complex map. Our results identified the antigenic epitope that had significant overlap with the receptor binding site, and the structures revealed that binding of Fab induced conformational changes to the virus. We were also able to assign the order and position of attached Fabs to allow assessment of complementarity between the Fabs bound to different positions. This approach therefore provides a method for using cryo-EM to investigate complementarity of antibody binding.

PubMed: 34074770
DOI: 10.1073/pnas.2025452118

実験手法

ELECTRON MICROSCOPY (2.4 Å)