7M2C

Solution NMR Structure of PawL-Derived Peptide PLP-46

7M2C の概要

• エントリーDOI: 10.2210/pdb7m2c/pdb
• 関連するPDBエントリー: 7M25 7M27 7M28 7M29 7M2A 7M2B
• NMR情報: BMRB: 30887
• 分子名称: PawL-Derived Peptide PLP-46 (1 entity in total)
• 機能のキーワード: cyclic, plp, orbitide, aep-processed, plant protein
• 由来する生物種: Parthenium argentatum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 777.86

構造登録者

Payne, C.D.,Rosengren, K.J. (登録日: 2021-03-16, 公開日: 2021-03-24, 最終更新日: 2024-11-13)

主引用文献

Payne, C.D.,Fisher, M.F.,Mylne, J.S.,Rosengren, K.J.
Structural Characterization of the PawL-Derived Peptide Family, an Ancient Subfamily of Orbitides.
J.Nat.Prod., 84:2914-2922, 2021

PubMed Abstract: Plants are an excellent source of bioactive peptides, often with disulfide bonds and/or a cyclic backbone. While focus has predominantly been directed at disulfide-rich peptides, a large family of small, cyclic plant peptides lacking disulfide bonds, known as orbitides, has been relatively ignored. A recently discovered subfamily of orbitides is the PawL-derived peptides (PLPs), produced during the maturation of precursors for seed storage albumins. Although their evolutionary origins have been dated, in-depth exploration of the family's structural characteristics and potential bioactivities remains to be conducted. Here we present an extensive and systematic characterization of the PLP family. Nine PLPs were chosen and prepared by solid phase peptide synthesis. Their structural features were studied using solution NMR spectroscopy, and seven were found to possess regions of backbone order. Ordered regions consist of β-turns, with some PLPs adopting two well-defined β-turns within sequences as short as seven residues, which are largely the result of side chain interactions. Our data highlight that the sequence diversity within this family results in equally diverse structures. None of these nine PLPs showed antibacterial or antifungal activity.

PubMed: 34672199
DOI: 10.1021/acs.jnatprod.1c00672

実験手法

SOLUTION NMR