7LTU

AALALL SEGMENT FROM THE NUCLEOPROTEIN OF SARS-COV-2, RESIDUES 217-222, CRYSTAL FORM 1

7LTU の概要

• エントリーDOI: 10.2210/pdb7ltu/pdb
• 分子名称: AALALL SEGMENT FROM THE NUCLEOPROTEIN OF SARS-COV-2,RESIDUES 217-222, trifluoroacetic acid (3 entities in total)
• 機能のキーワード: amyloid fibril, protein fibril
• 由来する生物種: SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (2019-NCOV, SARS-COV-2)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 1483.51

構造登録者

Zee, C.-T.,Sawaya, M.R.,Rodriguez, J.A.,Eisenberg, D.S. (登録日: 2021-02-20, 公開日: 2021-03-17, 最終更新日: 2024-04-03)

主引用文献

Tayeb-Fligelman, E.,Cheng, X.,Tai, C.,Bowler, J.T.,Griner, S.,Sawaya, M.R.,Seidler, P.M.,Jiang, Y.X.,Lu, J.,Rosenberg, G.M.,Salwinski, L.,Abskharon, R.,Zee, C.T.,Hou, K.,Li, Y.,Boyer, D.R.,Murray, K.A.,Falcon, G.,Anderson, D.H.,Cascio, D.,Saelices, L.,Damoiseaux, R.,Guo, F.,Eisenberg, D.S.
Inhibition of amyloid formation of the Nucleoprotein of SARS-CoV-2.
Biorxiv, 2021

PubMed Abstract: The SARS-CoV-2 Nucleoprotein (NCAP) functions in RNA packaging during viral replication and assembly. Computational analysis of its amino acid sequence reveals a central low-complexity domain (LCD) having sequence features akin to LCDs in other proteins known to function in liquid-liquid phase separation. Here we show that in the presence of viral RNA, NCAP, and also its LCD segment alone, form amyloid-like fibrils when undergoing liquid-liquid phase separation. Within the LCD we identified three 6-residue segments that drive amyloid fibril formation. We determined atomic structures for fibrils formed by each of the three identified segments. These structures informed our design of peptide inhibitors of NCAP fibril formation and liquid-liquid phase separation, suggesting a therapeutic route for Covid-19.

PubMed: 33688654
DOI: 10.1101/2021.03.05.434000

実験手法

X-RAY DIFFRACTION (1.122 Å)