7LNS

NMR solution structure of PsDef2 defensin from P. sylvestris

7LNS の概要

• エントリーDOI: 10.2210/pdb7lns/pdb
• NMR情報: BMRB: 30854
• 分子名称: Defensin-2 (1 entity in total)
• 機能のキーワード: antifungal protein, antibacterial protein, plant protein
• 由来する生物種: Pinus sylvestris (Scots pine)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5645.63

構造登録者

Nesmelova, I.V.,Kovaleva, V.,Hrunyk, N.I.,Yusypovych, Y.M.,Shalovylo, Y.I. (登録日: 2021-02-08, 公開日: 2022-03-30, 最終更新日: 2024-10-23)

主引用文献

Bukhteeva, I.,Hrunyk, N.I.,Yusypovych, Y.M.,Shalovylo, Y.I.,Kovaleva, V.,Nesmelova, I.V.
Structure, dynamics, and function of PsDef2 defensin from Pinus sylvestris.
Structure, 30:753-, 2022

PubMed Abstract: Plant defensins demonstrate high structural stability at extreme temperatures and pH values and, in general, are non-toxic to mammalian cells. These properties make them attractive candidates for use in biotechnology and biomedicine. Knowing the structure-function relationship is desirable to guide the design of plant defensin-based applications. Thus far, the broad range of biological activities was described only for one defensin from gymnosperms, the defensin PsDef1 from Scots pine. Here, we report that closely related defensin from the same taxonomy group, PsDef2, differing from PsDef1 by six amino acids, also possesses antimicrobial, antibacterial, and insect α-amylase inhibitory activities. We also report the solution structure and dynamics properties of PsDef2 assessed using a combination of experimental nuclear magnetic resonance (NMR) techniques. Lastly, we perform a comparative analysis of PsDef2 and PsDef1 gaining a molecular-level insight into their structure-dynamics-function relationship.

PubMed: 35334207
DOI: 10.1016/j.str.2022.03.001

実験手法

SOLUTION NMR