7LNK

Gorilla Bocavirus 1 Capsid

これはPDB形式変換不可エントリーです。

7LNK の概要

• エントリーDOI: 10.2210/pdb7lnk/pdb
• EMDBエントリー: 23460
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: icosahedral capsid, gorilla bocavirus, gbov, virus, parvovirus
• 由来する生物種: Bocavirus gorilla/GBoV1/2009
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 3452894.76

構造登録者

Yu, J.C.,Mietzsch, M.,Agbandje-McKenna, M. (登録日: 2021-02-07, 公開日: 2021-03-17, 最終更新日: 2024-03-06)

主引用文献

Yu, J.C.,Mietzsch, M.,Singh, A.,Jimenez Ybargollin, A.,Kailasan, S.,Chipman, P.,Bhattacharya, N.,Fakhiri, J.,Grimm, D.,Kapoor, A.,Kucinskaite-Kodze, I.,Zvirbliene, A.,Soderlund-Venermo, M.,McKenna, R.,Agbandje-McKenna, M.
Characterization of the GBoV1 Capsid and Its Antibody Interactions.
Viruses, 13:-, 2021

PubMed Abstract: Human bocavirus 1 (HBoV1) has gained attention as a gene delivery vector with its ability to infect polarized human airway epithelia and 5.5 kb genome packaging capacity. Gorilla bocavirus 1 (GBoV1) VP3 shares 86% amino acid sequence identity with HBoV1 but has better transduction efficiency in several human cell types. Here, we report the capsid structure of GBoV1 determined to 2.76 Å resolution using cryo-electron microscopy (cryo-EM) and its interaction with mouse monoclonal antibodies (mAbs) and human sera. GBoV1 shares capsid surface morphologies with other parvoviruses, with a channel at the 5-fold symmetry axis, protrusions surrounding the 3-fold axis and a depression at the 2-fold axis. A 2/5-fold wall separates the 2-fold and 5-fold axes. Compared to HBoV1, differences are localized to the 3-fold protrusions. Consistently, native dot immunoblots and cryo-EM showed cross-reactivity and binding, respectively, by a 5-fold targeted HBoV1 mAb, 15C6. Surprisingly, recognition was observed for one out of three 3-fold targeted mAbs, 12C1, indicating some structural similarity at this region. In addition, GBoV1, tested against 40 human sera, showed the similar rates of seropositivity as HBoV1. Immunogenic reactivity against parvoviral vectors is a significant barrier to efficient gene delivery. This study is a step towards optimizing bocaparvovirus vectors with antibody escape properties.

PubMed: 33672786
DOI: 10.3390/v13020330

実験手法

ELECTRON MICROSCOPY (2.76 Å)