7LHL

cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain

7LHL の概要

• エントリーDOI: 10.2210/pdb7lhl/pdb
• EMDBエントリー: 23345
• 分子名称: ATP-dependent DNA helicase (1 entity in total)
• 機能のキーワード: helicase, winged helix, hydrolase
• 由来する生物種: Mycolicibacterium smegmatis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 648038.25

構造登録者

Wang, J.,Warren, G.M.,Shuman, S.,Patel, D.J. (登録日: 2021-01-25, 公開日: 2021-04-07, 最終更新日: 2025-05-14)

主引用文献

Warren, G.M.,Wang, J.,Patel, D.J.,Shuman, S.
Oligomeric quaternary structure of Escherichia coli and Mycobacterium smegmatis Lhr helicases is nucleated by a novel C-terminal domain composed of five winged-helix modules.
Nucleic Acids Res., 49:3876-3887, 2021

PubMed Abstract: Mycobacterium smegmatis Lhr (MsmLhr; 1507-aa) is the founder of a novel clade of bacterial helicases. MsmLhr consists of an N-terminal helicase domain (aa 1-856) with a distinctive tertiary structure (Lhr-Core) and a C-terminal domain (Lhr-CTD) of unknown structure. Here, we report that Escherichia coli Lhr (EcoLhr; 1538-aa) is an ATPase, translocase and ATP-dependent helicase. Like MsmLhr, EcoLhr translocates 3' to 5' on ssDNA and unwinds secondary structures en route, with RNA:DNA hybrid being preferred versus DNA:DNA duplex. The ATPase and translocase activities of EcoLhr inhere to its 877-aa Core domain. Full-length EcoLhr and MsmLhr have homo-oligomeric quaternary structures in solution, whereas their respective Core domains are monomers. The MsmLhr CTD per se is a homo-oligomer in solution. We employed cryo-EM to solve the structure of the CTD of full-length MsmLhr. The CTD protomer is composed of a series of five winged-helix (WH) modules and a β-barrel module. The CTD adopts a unique homo-tetrameric quaternary structure. A Lhr-CTD subdomain, comprising three tandem WH modules and the β-barrel, is structurally homologous to AlkZ, a bacterial DNA glycosylase that recognizes and excises inter-strand DNA crosslinks. This homology is noteworthy given that Lhr is induced in mycobacteria exposed to the inter-strand crosslinker mitomycin C.

PubMed: 33744958
DOI: 10.1093/nar/gkab145

実験手法

ELECTRON MICROSCOPY (3.6 Å)