7LGF

Asymmetric unit for phage Qbeta prolate particle

7LGF の概要

• エントリーDOI: 10.2210/pdb7lgf/pdb
• EMDBエントリー: 23322
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: bacteriophage, virus, qbeta, prolate, virus like particle
• 由来する生物種: Escherichia phage Qbeta (Bacteriophage Q-beta)
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 299629.49

構造登録者

Chang, J.Y.,Zhang, J. (登録日: 2021-01-20, 公開日: 2022-01-26, 最終更新日: 2024-10-09)

主引用文献

Chang, J.Y.,Gorzelnik, K.V.,Thongchol, J.,Zhang, J.
Structural Assembly of Q beta Virion and Its Diverse Forms of Virus-like Particles.
Viruses, 14:-, 2022

PubMed Abstract: The coat proteins (CPs) of single-stranded RNA bacteriophages (ssRNA phages) directly assemble around the genomic RNA (gRNA) to form a near-icosahedral capsid with a single maturation protein (Mat) that binds the gRNA and interacts with the retractile pilus during infection of the host. Understanding the assembly of ssRNA phages is essential for their use in biotechnology, such as RNA protection and delivery. Here, we present the complete gRNA model of the ssRNA phage Qβ, revealing that the 3' untranslated region binds to the Mat and the 4127 nucleotides fold domain-by-domain, and is connected through long-range RNA-RNA interactions, such as kissing loops. Thirty-three operator-like RNA stem-loops are located and primarily interact with the asymmetric A/B CP-dimers, suggesting a pathway for the assembly of the virions. Additionally, we have discovered various forms of the virus-like particles (VLPs), including the canonical = 3 icosahedral, larger = 4 icosahedral, prolate, oblate forms, and a small prolate form elongated along the 3-fold axis. These particles are all produced during a normal infection, as well as when overexpressing the CPs. When overexpressing the shorter RNA fragments encoding only the CPs, we observed an increased percentage of the smaller VLPs, which may be sufficient to encapsidate a shorter RNA.

PubMed: 35215818
DOI: 10.3390/v14020225

実験手法

ELECTRON MICROSCOPY (6.1 Å)