7LAG

CRYSTAL STRUCTURE OF MYELOPEROXIDASE SUBFORM C (MPO) COMPLEX WITH Compound-14 AKA 7-({1-[(3-phenoxyphenyl)methyl]-1H-pyrazol-4-yl}methyl)-3H-[1,2,3]triazolo[4,5-b]pyridin-5-amine

Summary for 7LAG

• Entry DOI: 10.2210/pdb7lag/pdb
• Descriptor: Myeloperoxidase light chain, Isoform H14 of Myeloperoxidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: myeloperoxidase, mpo, oxidoreductase, heme-dependent peroxidase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 8
• Total formula weight: 271310.93

Authors

Khan, J.A. (deposition date: 2021-01-06, release date: 2021-04-21, Last modification date: 2024-10-23)

Primary citation

Hu, C.H.,Neissel Valente, M.W.,Halpern, O.S.,Jusuf, S.,Khan, J.A.,Locke, G.A.,Duke, G.J.,Liu, X.,Duclos, F.J.,Wexler, R.R.,Kick, E.K.,Smallheer, J.M.
Small molecule and macrocyclic pyrazole derived inhibitors of myeloperoxidase (MPO).
Bioorg.Med.Chem.Lett., 42:128010-128010, 2021

PubMed Abstract: Myeloperoxidase (MPO), a critical enzyme in antimicrobial host-defense, has been implicated in chronic inflammatory diseases such as coronary artery disease. The design and evaluation of MPO inhibitors for the treatment of cardiovascular disease are reported herein. Starting with the MPO and triazolopyridine 3 crystal structure, novel inhibitors were designed incorporating a substituted pyrazole, which allowed for substituents to interact with hydrophobic and hydrophilic patches in the active site. SAR exploration of the substituted pyrazoles led to piperidine 17, which inhibited HOCl production from activated neutrophils with an IC value of 2.4 μM and had selectivity against thyroid peroxidase (TPO). Optimization of alkylation chemistry on the pyrazole nitrogen facilitated the preparation of many analogs, including macrocycles designed to bridge two hydrophobic regions of the active site. Multiple macrocyclization strategies were pursued to prepare analogs that optimally bound to the active site, leading to potent macrocyclic MPO inhibitors with TPO selectivity, such as compound 30.

PubMed: 33811992
DOI: 10.1016/j.bmcl.2021.128010

Experimental method

X-RAY DIFFRACTION (2.85 Å)