7L52

Crystal Structure of the Metallo Beta Lactamase L1 from Stenotrophomonas maltophilia Determined by Serial Crystallography

7L52 の概要

• エントリーDOI: 10.2210/pdb7l52/pdb
• 関連するPDBエントリー: 6UA1
• 分子名称: Putative metallo-beta-lactamase l1 (Beta-lactamase type ii) (Ec 3.5.2.6) (Penicillinase), ZINC ION (3 entities in total)
• 機能のキーワード: metallo beta lactamase, serial crystallography, structural genomics, center for structural genomics of infectious diseases, hydrolase, csgid
• 由来する生物種: Stenotrophomonas maltophilia (strain K279a)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29374.76

構造登録者

Wilamowski, M.,Kim, Y.,Sherrell, D.A.,Lavens, A.,Maltseva, N.,Endres, M.,Babnigg, G.,Joachimiak, A.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2020-12-21, 公開日: 2020-12-30, 最終更新日: 2026-03-25)

主引用文献

Sherrell, D.A.,Lavens, A.,Wilamowski, M.,Kim, Y.,Chard, R.,Lazarski, K.,Rosenbaum, G.,Vescovi, R.,Johnson, J.L.,Akins, C.,Chang, C.,Michalska, K.,Babnigg, G.,Foster, I.,Joachimiak, A.
Fixed-target serial crystallography at the Structural Biology Center.
J.Synchrotron Radiat., 29:1141-1151, 2022

PubMed Abstract: Serial synchrotron crystallography enables the study of protein structures under physiological temperature and reduced radiation damage by collection of data from thousands of crystals. The Structural Biology Center at Sector 19 of the Advanced Photon Source has implemented a fixed-target approach with a new 3D-printed mesh-holder optimized for sample handling. The holder immobilizes a crystal suspension or droplet emulsion on a nylon mesh, trapping and sealing a near-monolayer of crystals in its mother liquor between two thin Mylar films. Data can be rapidly collected in scan mode and analyzed in near real-time using piezoelectric linear stages assembled in an XYZ arrangement, controlled with a graphical user interface and analyzed using a high-performance computing pipeline. Here, the system was applied to two β-lactamases: a class D serine β-lactamase from Chitinophaga pinensis DSM 2588 and L1 metallo-β-lactamase from Stenotrophomonas maltophilia K279a.

PubMed: 36073872
DOI: 10.1107/S1600577522007895

実験手法

X-RAY DIFFRACTION (1.85 Å)