7L1F

SARS-CoV-2 RdRp in complex with 4 Remdesivir monophosphate

7L1F の概要

• エントリーDOI: 10.2210/pdb7l1f/pdb
• EMDBエントリー: 23109
• 分子名称: RNA-directed RNA polymerase, Non-structural protein 8, Non-structural protein 7, ... (5 entities in total)
• 機能のキーワード: viral protein, viral protein-rna complex, viral protein/rna
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 134214.74

構造登録者

Bravo, J.P.K.,Taylor, D.W. (登録日: 2020-12-14, 公開日: 2021-02-10, 最終更新日: 2024-03-06)

主引用文献

Bravo, J.P.K.,Dangerfield, T.L.,Taylor, D.W.,Johnson, K.A.
Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication.
Mol.Cell, 81:1548-1552.e4, 2021

PubMed Abstract: Remdesivir is a nucleoside analog approved by the US FDA for treatment of COVID-19. Here, we present a 3.9-Å-resolution cryo-EM reconstruction of a remdesivir-stalled RNA-dependent RNA polymerase complex, revealing full incorporation of 3 copies of remdesivir monophosphate (RMP) and a partially incorporated fourth RMP in the active site. The structure reveals that RMP blocks RNA translocation after incorporation of 3 bases following RMP, resulting in delayed chain termination, which can guide the rational design of improved antiviral drugs.

PubMed: 33631104
DOI: 10.1016/j.molcel.2021.01.035

実験手法

ELECTRON MICROSCOPY (3.89 Å)