7KZV

Structure of the human fanconi anaemia Core-UBE2T-ID-DNA complex in closed state

これはPDB形式変換不可エントリーです。

7KZV の概要

• エントリーDOI: 10.2210/pdb7kzv/pdb
• EMDBエントリー: 23090
• 分子名称: Fanconi anemia group A protein, Ubiquitin-conjugating enzyme E2 T, Fanconi anemia, complementation group I, ... (15 entities in total)
• 機能のキーワード: complex, ligase, ligase-dna complex, ligase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 1506427.30

構造登録者

Wang, S.L.,Pavletich, N.P. (登録日: 2020-12-10, 公開日: 2021-03-10, 最終更新日: 2024-11-20)

主引用文献

Wang, S.,Wang, R.,Peralta, C.,Yaseen, A.,Pavletich, N.P.
Structure of the FA core ubiquitin ligase closing the ID clamp on DNA.
Nat.Struct.Mol.Biol., 28:300-309, 2021

PubMed Abstract: The Fanconi anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks. Central to the pathway is the FA core complex, a ubiquitin ligase of nine subunits that monoubiquitinates the FANCI-FANCD2 (ID) DNA clamp. The 3.1 Å structure of the 1.1-MDa human FA core complex, described here, reveals an asymmetric assembly with two copies of all but the FANCC, FANCE and FANCF subunits. The asymmetry is crucial, as it prevents the binding of a second FANCC-FANCE-FANCF subcomplex that inhibits the recruitment of the UBE2T ubiquitin conjugating enzyme, and instead creates an ID binding site. A single active site then ubiquitinates FANCD2 and FANCI sequentially. We also present the 4.2-Å structures of the human core-UBE2T-ID-DNA complex in three conformations captured during monoubiquitination. They reveal the core-UBE2T complex remodeling the ID-DNA complex, closing the clamp on the DNA before ubiquitination. Monoubiquitination then prevents clamp opening after release from the core.

PubMed: 33686268
DOI: 10.1038/s41594-021-00568-8

実験手法

ELECTRON MICROSCOPY (4.2 Å)