7KZ8

Crystal structure of substrate-binding protein Aapf from Pseudomonas sp. PDC86

7KZ8 の概要

• エントリーDOI: 10.2210/pdb7kz8/pdb
• 分子名称: Peptide/nickel transport system substrate-binding protein AapF, GLYCEROL (3 entities in total)
• 機能のキーワード: substrate-binding protein (sbps), abc-transporter, type-c sbps, transport protein
• 由来する生物種: Pseudomonas sp. PDC86
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55274.11

構造登録者

Luo, S.,Dadhwal, P.,Tong, L. (登録日: 2020-12-10, 公開日: 2021-03-17, 最終更新日: 2023-10-18)

主引用文献

Luo, S.,Coutinho, B.G.,Dadhwal, P.,Oda, Y.,Ren, J.,Schaefer, A.L.,Greenberg, E.P.,Harwood, C.S.,Tong, L.
Structural basis for a bacterial Pip system plant effector recognition protein.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: A number of plant-associated proteobacteria have LuxR family transcription factors that we refer to as PipR subfamily members. PipR proteins play roles in interactions between bacteria and their plant hosts, and some are important for bacterial virulence of plants. We identified an ethanolamine derivative, -(2-hydroxyethyl)-2-(2-hydroxyethylamino) acetamide (HEHEAA), as a potent effector of PipR-mediated gene regulation in the plant endophyte GM79. HEHEAA-dependent PipR activity requires an ATP-binding cassette-type active transport system, and the periplasmic substrate-binding protein (SBP) of that system binds HEHEAA. To begin to understand the molecular basis of PipR system responses to plant factors we crystallized a HEHEAA-responsive SBP in the free- and HEHEAA-bound forms. The SBP, which is similar to peptide-binding SBPs, was in a closed conformation. A narrow cavity at the interface of its two lobes is wide enough to bind HEHEAA, but it cannot accommodate peptides with side chains. The polar atoms of HEHEAA are recognized by hydrogen-bonding interactions, and additional SBP residues contribute to the binding site. This binding mode was confirmed by a structure-based mutational analysis. We also show that a closely related SBP from the plant pathogen pv DC3000 does not recognize HEHEAA. However, a single amino acid substitution in the presumed effector-binding pocket of the SBP converted it to a weak HEHEAA-binding protein. The PipR depends on a plant effector for activity, and our findings imply that different PipR-associated SBPs bind different effectors.

PubMed: 33649224
DOI: 10.1073/pnas.2019462118

実験手法

X-RAY DIFFRACTION (2.2 Å)