7KYW

Crystal structure of timothy grass allergen Phl p 12.0101 reveals an unusual profilin dimer

7KYW の概要

• エントリーDOI: 10.2210/pdb7kyw/pdb
• 分子名称: Profilin-1, CITRIC ACID (3 entities in total)
• 機能のキーワード: grass allergy, allergen, profilin, phleum pratense, pollen-food syndrome, oral allergy syndrome
• 由来する生物種: Phleum pratense (Common timothy)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14368.30

構造登録者

O'Malley, A.,Kapingidza, A.B.,Hyduke, N.,Dolamore, C.,Chruszcz, M. (登録日: 2020-12-09, 公開日: 2021-03-31, 最終更新日: 2024-11-06)

主引用文献

O'Malley, A.,Kapingidza, A.B.,Hyduke, N.,Dolamore, C.,Kowal, K.,Chruszcz, M.
Crystal structure of timothy grass allergen Phl p 12.0101 reveals an unusual profilin dimer.
Acta Biochim.Pol., 68:15-22, 2021

PubMed Abstract: Timothy grass pollen is a source of potent allergens. Among them, Phl p 1 and Phl p 5 are thought to be the most important, as a majority of timothy grass-allergic individuals have IgE antibodies directed against these two allergens. The profilin from timothy grass (Phl p 12) has been registered as a minor allergen, with up to 35% of individuals in populations of grass pollen allergic patients showing IgE binding to Phl p 12. Profilins are primarily minor allergens and are known for a high likelihood of co-sensitization as well as cross-reactivity situations caused by their sequence and structure similarity. The crystal structure of Phl p 12.0101 was determined and it revealed that this allergen may form an unusual dimer not previously observed among any profilins. For example, the Phl p 12 dimer has a completely different geometry and interface when compared with the latex profilin (Hev b 8) dimer that has its crystal structure determined. The structure of Phl p 12.0101 is described in the context of allergenic sensitization and allergy diagnostics. Moreover, the structure of the Phl p 12.0101 dimer is discussed, taking into account the production of recombinant allergens and their storage.

PubMed: 33720678
DOI: 10.18388/abp.2020_5587

実験手法

X-RAY DIFFRACTION (2.3 Å)