7KWG

Staphylococcus aureus 30S ribosomal subunit in presence of spermidine

7KWG の概要

• エントリーDOI: 10.2210/pdb7kwg/pdb
• EMDBエントリー: 23052
• 分子名称: mRNA, 30S ribosomal protein S9, 30S ribosomal protein S10, ... (21 entities in total)
• 機能のキーワード: pathogen, small ribosomal subunit, spermidine, ribosome
• 由来する生物種: Staphylococcus aureus subsp. aureus NCTC 8325; 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 788854.46

構造登録者

Belinite, M.,Khusainov, I.,Marzi, S.,Romby, P.,Yusupov, M.,Hashem, Y. (登録日: 2020-11-30, 公開日: 2021-01-27, 最終更新日: 2024-05-29)

主引用文献

Belinite, M.,Khusainov, I.,Soufari, H.,Marzi, S.,Romby, P.,Yusupov, M.,Hashem, Y.
Stabilization of Ribosomal RNA of the Small Subunit by Spermidine in Staphylococcus aureus .
Front Mol Biosci, 8:738752-738752, 2021

PubMed Abstract: Cryo-electron microscopy is now used as a method of choice in structural biology for studying protein synthesis, a process mediated by the ribosome machinery. In order to achieve high-resolution structures using this approach, one needs to obtain homogeneous and stable samples, which requires optimization of ribosome purification in a species-dependent manner. This is especially critical for the bacterial small ribosomal subunit that tends to be unstable in the absence of ligands. Here, we report a protocol for purification of stable 30 S from the Gram-positive bacterium and its cryo-EM structures: in presence of spermidine at a resolution ranging between 3.4 and 3.6 Å and in its absence at 5.3 Å. Using biochemical characterization and cryo-EM, we demonstrate the importance of spermidine for stabilization of the 30 S preserving favorable conformation of the helix 44.

PubMed: 34869582
DOI: 10.3389/fmolb.2021.738752

実験手法

ELECTRON MICROSCOPY (3.75 Å)