7KVB

Chimeric flavivirus between Binjari virus and Murray Valley encephalitis virus

7KVB の概要

• エントリーDOI: 10.2210/pdb7kvb/pdb
• EMDBエントリー: 23043 23044 23045
• 分子名称: Envelope protein E, Matrix protein M, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: flavivirus, glycoprotein, entry, fusion, virus
• 由来する生物種: Murray Valley encephalitis virus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 186769.78

構造登録者

Hardy, J.M.,Venugopal, H.V.,Newton, N.D.,Watterson, D.,Coulibaly, F.J. (登録日: 2020-11-27, 公開日: 2020-12-23, 最終更新日: 2024-11-06)

主引用文献

Hardy, J.M.,Newton, N.D.,Modhiran, N.,Scott, C.A.P.,Venugopal, H.,Vet, L.J.,Young, P.R.,Hall, R.A.,Hobson-Peters, J.,Coulibaly, F.,Watterson, D.
A unified route for flavivirus structures uncovers essential pocket factors conserved across pathogenic viruses.
Nat Commun, 12:3266-3266, 2021

PubMed Abstract: The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat these pathogens. Here, we present a chimeric platform based on an insect-specific flavivirus for the safe and rapid structural analysis of pathogenic viruses. We use this approach to resolve the architecture of two neurotropic viruses and a structure of dengue virus at 2.5  Å, the highest resolution for an enveloped virion. These reconstructions allow improved modelling of the stem region of the envelope protein, revealing two lipid-like ligands within highly conserved pockets. We show that these sites are essential for viral growth and important for viral maturation. These findings define a hallmark of flavivirus virions and a potential target for broad-spectrum antivirals and vaccine design. We anticipate the chimeric platform to be widely applicable for investigating flavivirus biology.

PubMed: 34075032
DOI: 10.1038/s41467-021-22773-1

実験手法

ELECTRON MICROSCOPY (3.7 Å)