7KKR

Fluoride channel Fluc-Ec2 wild-type with bromide

7KKR の概要

• エントリーDOI: 10.2210/pdb7kkr/pdb
• 分子名称: Putative fluoride ion transporter CrcB, monobody, DECYL-BETA-D-MALTOPYRANOSIDE, ... (5 entities in total)
• 機能のキーワード: fluoride channel, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49612.16

構造登録者

McIlwain, B.C.,Stockbridge, R.B. (登録日: 2020-10-28, 公開日: 2021-08-04, 最終更新日: 2023-10-18)

主引用文献

McIlwain, B.C.,Gundepudi, R.,Koff, B.B.,Stockbridge, R.B.
The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.
Elife, 10:-, 2021

PubMed Abstract: Fluc family fluoride channels protect microbes against ambient environmental fluoride by undermining the cytoplasmic accumulation of this toxic halide. These proteins are structurally idiosyncratic, and thus the permeation pathway and mechanism have no analogy in other known ion channels. Although fluoride-binding sites were identified in previous structural studies, it was not evident how these ions access aqueous solution, and the molecular determinants of anion recognition and selectivity have not been elucidated. Using x-ray crystallography, planar bilayer electrophysiology, and liposome-based assays, we identified additional binding sites along the permeation pathway. We used this information to develop an oriented system for planar lipid bilayer electrophysiology and observed anion block at one of these sites, revealing insights into the mechanism of anion recognition. We propose a permeation mechanism involving alternating occupancy of anion-binding sites that are fully assembled only as the substrate approaches.

PubMed: 34250906
DOI: 10.7554/eLife.69482

実験手法

X-RAY DIFFRACTION (3.11 Å)