7KKI

Crystal structure of the MarR family transcriptional regulator from Variovorax paradoxus bound to 2,4-Dichlorophenoxyacetic acid

7KKI の概要

• エントリーDOI: 10.2210/pdb7kki/pdb
• 分子名称: Transcriptional regulator, MarR family, SULFATE ION, (2,4-DICHLOROPHENOXY)ACETIC ACID, ... (4 entities in total)
• 機能のキーワード: transcriptional regulator, ligand binding, dna binding protein
• 由来する生物種: Variovorax paradoxus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18638.95

構造登録者

Walton, W.G.,Redinbo, M.R.,Dangl, J.L. (登録日: 2020-10-27, 公開日: 2021-12-01, 最終更新日: 2023-10-25)

主引用文献

Conway, J.M.,Walton, W.G.,Salas-Gonzalez, I.,Law, T.F.,Lindberg, C.A.,Crook, L.E.,Kosina, S.M.,Fitzpatrick, C.R.,Lietzan, A.D.,Northen, T.R.,Jones, C.D.,Finkel, O.M.,Redinbo, M.R.,Dangl, J.L.
Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.
Nat Microbiol, 7:1817-1833, 2022

PubMed Abstract: Chemical signalling in the plant microbiome can have drastic effects on microbial community structure, and on host growth and development. Previously, we demonstrated that the auxin metabolic signal interference performed by the bacterial genus Variovorax via an auxin degradation locus was essential for maintaining stereotypic root development in an ecologically relevant bacterial synthetic community. Here, we dissect the Variovorax auxin degradation locus to define the genes iadDE as necessary and sufficient for indole-3-acetic acid (IAA) degradation and signal interference. We determine the crystal structures and binding properties of the operon's MarR-family repressor with IAA and other auxins. Auxin degradation operons were identified across the bacterial tree of life and we define two distinct types on the basis of gene content and metabolic products: iac-like and iad-like. The structures of MarRs from representatives of each auxin degradation operon type establish that each has distinct IAA-binding pockets. Comparison of representative IAA-degrading strains from diverse bacterial genera colonizing Arabidopsis plants show that while all degrade IAA, only strains containing iad-like auxin-degrading operons interfere with auxin signalling in a complex synthetic community context. This suggests that iad-like operon-containing bacterial strains, including Variovorax species, play a key ecological role in modulating auxins in the plant microbiome.

PubMed: 36266335
DOI: 10.1038/s41564-022-01244-3

実験手法

X-RAY DIFFRACTION (1.6 Å)