7KGC

Crystal structure of a perchloric acid-soluble protein (PSP) from Trichomonas vaginalis at 1.95 A

Summary for 7KGC

• Entry DOI: 10.2210/pdb7kgc/pdb
• Related: 1QAH
• Descriptor: Putative translation initiation inhibitor, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• Functional Keywords: trichomonas vaginalis, perchloric acid-soluble protein, rna nuclease, hydrolase
• Biological source: Trichomonas vaginalis
• Total number of polymer chains: 4
• Total formula weight: 54172.41

Authors

Diaz-Vilchis, A.,Rudino-Pinera, E.,Alvarez-Sanchez, M.E.,Arreola, R. (deposition date: 2020-10-16, release date: 2021-10-20, Last modification date: 2024-10-16)

Primary citation

Millan-Pacheco, C.,Arreola, R.,Villalobos-Osnaya, A.,Garza-Ramos, G.,Serratos, I.N.,Diaz-Vilchis, A.,Rudino-Pinera, E.,Alvarez-Sanchez, M.E.
A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis.
Pathogens, 12:-, 2023

PubMed Abstract: To understand whether protein Tv-PSP1 from recognizes mRNA parasite stem-loop structures, we conducted REMSA and intrinsic fluorescence assays. We found the recombinant Tv-PSP1 structure, determined with X-ray crystallography, showed unusual thermal stability of the quaternary structure, associated with a disulfide bridge CYS76-CYS104. To gain deeper insight into the Tv-PSP1 interaction with mRNA stem-loops (mRNAsl) and its relationship with thermal stability, we also used an integrated computational protocol that combined molecular dynamics simulations, docking assays, and binding energy calculations. Docking models allowed us to determine a putative contact surface interaction region between Tv-PSP1 and mRNAsl. We determined the contributions of these complexes to the binding free energy (ΔG) in the electrostatic (ΔG) and nonelectrostatic (ΔG) components using the Adaptive Poisson-Boltzmann Solver (APBS) program. We are the first, to the best of our knowledge, to show the interaction between Tv-PSP1 and the stem-loop structures of mRNA.

PubMed: 36678426
DOI: 10.3390/pathogens12010079

Experimental method

X-RAY DIFFRACTION (1.95 Å)