7KGA

Crystal structure of a mosquito-borne flavivirus dumbbell RNA

7KGA の概要

• エントリーDOI: 10.2210/pdb7kga/pdb
• 分子名称: RNA (90-MER), IRIDIUM HEXAMMINE ION (3 entities in total)
• 機能のキーワード: viral rna, noncoding rna, flavivirus, viral replication, rna
• 由来する生物種: Donggang virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70922.18

構造登録者

Akiyama, B.M.,Graham, M.E.,O'Donoghue, Z.,Beckham, J.D.,Kieft, J.S. (登録日: 2020-10-16, 公開日: 2021-06-23, 最終更新日: 2024-03-06)

主引用文献

Akiyama, B.M.,Graham, M.E.,O Donoghue, Z.,Beckham, J.D.,Kieft, J.S.
Three-dimensional structure of a flavivirus dumbbell RNA reveals molecular details of an RNA regulator of replication.
Nucleic Acids Res., 49:7122-7138, 2021

PubMed Abstract: Mosquito-borne flaviviruses (MBFVs) including dengue, West Nile, yellow fever, and Zika viruses have an RNA genome encoding one open reading frame flanked by 5' and 3' untranslated regions (UTRs). The 3' UTRs of MBFVs contain regions of high sequence conservation in structured RNA elements known as dumbbells (DBs). DBs regulate translation and replication of the viral RNA genome, functions proposed to depend on the formation of an RNA pseudoknot. To understand how DB structure provides this function, we solved the x-ray crystal structure of the Donggang virus DB to 2.1Å resolution and used structural modeling to reveal the details of its three-dimensional fold. The structure confirmed the predicted pseudoknot and molecular modeling revealed how conserved sequences form a four-way junction that appears to stabilize the pseudoknot. Single-molecule FRET suggests that the DB pseudoknot is a stable element that can regulate the switch between translation and replication during the viral lifecycle by modulating long-range RNA conformational changes.

PubMed: 34133732
DOI: 10.1093/nar/gkab462

実験手法

X-RAY DIFFRACTION (2.1 Å)