Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

7KF6

Cryo-electron microscopy structure of the heavy metal efflux pump CusA in a homogeneous binding copper(1) state

7KF6 の概要
エントリーDOI10.2210/pdb7kf6/pdb
EMDBエントリー22844
分子名称Cation efflux system protein CusA, COPPER (I) ION (2 entities in total)
機能のキーワードefflux, pump, heavy metal. copper, silver, closed, open, transport, membrane protein, transport protein
由来する生物種Escherichia coli
タンパク質・核酸の鎖数3
化学式量合計347692.47
構造登録者
Moseng, M.A. (登録日: 2020-10-13, 公開日: 2021-04-14, 最終更新日: 2024-03-06)
主引用文献Moseng, M.A.,Lyu, M.,Pipatpolkai, T.,Glaza, P.,Emerson, C.C.,Stewart, P.L.,Stansfeld, P.J.,Yu, E.W.
Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export.
Mbio, 12:-, 2021
Cited by
PubMed Abstract: Gram-negative bacteria utilize the resistance-nodulation-cell division (RND) superfamily of efflux pumps to expel a variety of toxic compounds from the cell. The CusA membrane protein, which recognizes and extrudes biocidal Cu(I) and Ag(I) ions, belongs to the heavy-metal efflux (HME) subfamily of RND efflux pumps. We here report four structures of the trimeric CusA heavy-metal efflux pump in the presence of Cu(I) using single-particle cryo-electron microscopy (cryo-EM). We discover that different CusA protomers within the trimer are able to bind Cu(I) ions simultaneously. Our structural data combined with molecular dynamics (MD) simulations allow us to propose a mechanism for ion transport where each CusA protomer functions independently within the trimer. The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the trimer recognizes and extrudes Cu(I) independently.
PubMed: 33820823
DOI: 10.1128/mBio.00452-21
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.4 Å)
構造検証レポート
Validation report summary of 7kf6
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

PDB statisticsPDBj update infoContact PDBjnumon