7K7V

The X-ray crystal structure of SSR4, an S. pombe chromatin remodelling protein: iodide derivative

7K7V の概要

• エントリーDOI: 10.2210/pdb7k7v/pdb
• 分子名称: SWI/SNF and RSC complexes subunit ssr4, GLYCEROL, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: chromatin remodelling, sad phasing, novel structure, gene regulation
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22822.21

構造登録者

Peat, T.S.,Newman, J. (登録日: 2020-09-24, 公開日: 2020-12-16, 最終更新日: 2024-03-06)

主引用文献

Newman, J.,Nebl, T.,Van, H.,Peat, T.S.
The X-ray crystal structure of the N-terminal domain of Ssr4, a Schizosaccharomyces pombe chromatin-remodelling protein.
Acta Crystallogr.,Sect.F, 76:583-589, 2020

PubMed Abstract: Ssr4 is a yeast protein from Schizosaccharomyces pombe and is an essential part of the chromatin-remodelling [SWI/SNF and RSC (remodelling the structure of chromatin)] complexes found in S. pombe. These complexes (or their homologues) regulate gene expression in eukaryotic organisms, affecting a large number of genes both positively and negatively. The downstream effects are seen in development, and in humans have implications for disease such as cancer. The chromatin structure is altered by modifying the DNA-histone contacts, thus opening up or closing down sections of DNA to specific transcription factors that regulate the transcription of genes. The Ssr4 sequence has little homology to other sequences in the Protein Data Bank, so the structure was solved using an iodine derivative with SAD phasing. The structure of the N-terminal domain is an antiparallel β-sheet of seven strands with α-helices on one side and random coil on the other. The structure is significantly different to deposited structures and was used as a target in the most recent Critical Assessment of Techniques for Protein Structure Prediction (CASP; https://predictioncenter.org/) competition.

PubMed: 33263569
DOI: 10.1107/S2053230X20015216

実験手法

X-RAY DIFFRACTION (1.882 Å)