7K7A

Transmembrane structure of TNFR1

7K7A の概要

• エントリーDOI: 10.2210/pdb7k7a/pdb
• NMR情報: BMRB: 30799
• 分子名称: Tumor necrosis factor receptor superfamily member 1A (1 entity in total)
• 機能のキーワード: transmembrane helix, apoptosis
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 10113.33

構造登録者

Zhao, L.,Chou, J. (登録日: 2020-09-22, 公開日: 2020-09-30, 最終更新日: 2024-05-15)

主引用文献

Zhao, L.,Fu, Q.,Pan, L.,Piai, A.,Chou, J.J.
The Diversity and Similarity of Transmembrane Trimerization of TNF Receptors.
Front Cell Dev Biol, 8:569684-569684, 2020

PubMed Abstract: Receptors in the tumor necrosis factor receptor superfamily (TNFRSF) regulate proliferation of immune cells or induce programmed cell death, and many of them are candidates for antibody-based immunotherapy. Previous studies on several death receptors in the TNFRSF including Fas, p75NTR, and DR5 showed that the transmembrane helix (TMH) of these receptors can specifically oligomerize and their oligomeric states have direct consequences on receptor activation, suggesting a much more active role of TMH in receptor signaling than previously appreciated. Here, we report the structure of the TMH of TNFR1, another well studied member of the TNFRSF, in neutral bicelles that mimic a lipid bilayer. We find that TNFR1 TMH forms a defined trimeric complex in bicelles, and no evidences of higher-order clustering of trimers have been detected. Unexpectedly, a conserved proline, which is critical for Fas TMH trimerization, does not appear to play an important role in TNFR1 TMH trimerization, which is instead mediated by a glycine near the middle of the TMH. Further, TNFR1 TMH trimer shows a larger hydrophobic core than that of Fas or DR5, with four layers of hydrophobic interaction along the threefold axis. Comparison of the TNFR1 TMH structure with that of Fas and DR5 reveals reassuring similarities that have functional implications but also significant structural diversity that warrants systematic investigation of TMH oligomerization property for other members of the TNFRSF.

PubMed: 33163490
DOI: 10.3389/fcell.2020.569684

実験手法

SOLUTION NMR