7K5W

Cryo-EM structure of heterologous protein complex loaded Thermotoga maritima encapsulin capsid

7K5W の概要

• エントリーDOI: 10.2210/pdb7k5w/pdb
• EMDBエントリー: 22617
• 分子名称: Maritimacin (1 entity in total)
• 機能のキーワード: encapsulin, baculovirus expression system, cargo loading peptide, complex assembly, metal binding protein, hydrolase
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31489.79

構造登録者

Xiong, X.,Sun, C.,Vago, F.S.,Klose, T.,Zhu, J.,Jiang, W. (登録日: 2020-09-17, 公開日: 2020-10-07, 最終更新日: 2024-03-06)

主引用文献

Xiong, X.,Sun, C.,Vago, F.S.,Klose, T.,Zhu, J.,Jiang, W.
Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid.
Biomolecules, 10:-, 2020

PubMed Abstract: Encapsulin is a class of nanocompartments that is unique in bacteria and archaea to confine enzymatic activities and sequester toxic reaction products. Here we present a 2.87 Å resolution cryo-EM structure of encapsulin with heterologous protein complex loaded. It is the first successful case of expressing encapsulin and heterologous cargo protein in the insect cell system. Although we failed to reconstruct the cargo protein complex structure due to the signal interference of the capsid shell, we were able to observe some unique features of the cargo-loaded encapsulin shell, for example, an extra density at the fivefold pore that has not been reported before. These results would lead to a more complete understanding of the encapsulin cargo assembly process of .

PubMed: 32961724
DOI: 10.3390/biom10091342

実験手法

ELECTRON MICROSCOPY (2.87 Å)