7K37

Structure of full-length influenza HA with a head-binding antibody at pH 7.8

7K37 の概要

• エントリーDOI: 10.2210/pdb7k37/pdb
• EMDBエントリー: 22652
• 分子名称: Hemagglutinin, antibody Fab light chain, antibody Fab heavy chain, ... (8 entities in total)
• 機能のキーワード: influenza, virus, hemagglutinin, antibody, low ph, viral protein
• 由来する生物種: Influenza A virus (strain A/Hong Kong/1/1968 H3N2); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 356050.33

構造登録者

Gui, M.,Xiang, Y.,Gao, J. (登録日: 2020-09-10, 公開日: 2020-11-11, 最終更新日: 2024-10-23)

主引用文献

Gao, J.,Gui, M.,Xiang, Y.
Structural intermediates in the low pH-induced transition of influenza hemagglutinin.
Plos Pathog., 16:e1009062-e1009062, 2020

PubMed Abstract: The hemagglutinin (HA) glycoproteins of influenza viruses play a key role in binding host cell receptors and in mediating virus-host cell membrane fusion during virus infection. Upon virus entry, HA is triggered by low pH and undergoes large structural rearrangements from a prefusion state to a postfusion state. While structures of prefusion state and postfusion state of HA have been reported, the intermediate structures remain elusive. Here, we report two distinct low pH intermediate conformations of the influenza virus HA using cryo-electron microscopy (cryo-EM). Our results show that a decrease in pH from 7.8 to 5.2 triggers the release of fusion peptides from the binding pockets and then causes a dramatic conformational change in the central helices, in which the membrane-proximal ends of the central helices unwind to an extended form. Accompanying the conformational changes of the central helices, the stem region of the HA undergoes an anticlockwise rotation of 9.5 degrees and a shift of 15 Å. The HA head, after being stabilized by an antibody, remains unchanged compared to the neutral pH state. Thus, the conformational change of the HA stem region observed in our research is likely to be independent of the HA head. These results provide new insights into the structural transition of HA during virus entry.

PubMed: 33253316
DOI: 10.1371/journal.ppat.1009062

実験手法

ELECTRON MICROSCOPY (2.8 Å)