7K00

Structure of the Bacterial Ribosome at 2 Angstrom Resolution

これはPDB形式変換不可エントリーです。

7K00 の概要

• エントリーDOI: 10.2210/pdb7k00/pdb
• EMDBエントリー: 22586
• 分子名称: 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (62 entities in total)
• 機能のキーワード: antibiotics, post-translational modifications, post-transcriptional modifications, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 2209971.59

構造登録者

Watson, Z.L.,Ward, F.R.,Meheust, R.,Ad, O.,Schepartz, A.,Banfield, J.F.,Cate, J.H.D. (登録日: 2020-09-02, 公開日: 2020-09-23, 最終更新日: 2025-03-19)

主引用文献

Watson, Z.L.,Ward, F.R.,Meheust, R.,Ad, O.,Schepartz, A.,Banfield, J.F.,Cate, J.H.
Structure of the bacterial ribosome at 2 angstrom resolution.
Elife, 9:-, 2020

PubMed Abstract: Using cryo-electron microscopy (cryo-EM), we determined the structure of the 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous positioning of protein and RNA residues, their detailed chemical interactions, and chemical modifications. Notable features include the first examples of isopeptide and thioamide backbone substitutions in ribosomal proteins, the former likely conserved in all domains of life. The maps also reveal extensive solvation of the small (30S) ribosomal subunit, and interactions with A-site and P-site tRNAs, mRNA, and the antibiotic paromomycin. The maps and models of the bacterial ribosome presented here now allow a deeper phylogenetic analysis of ribosomal components including structural conservation to the level of solvation. The high quality of the maps should enable future structural analyses of the chemical basis for translation and aid the development of robust tools for cryo-EM structure modeling and refinement.

PubMed: 32924932
DOI: 10.7554/eLife.60482

実験手法

ELECTRON MICROSCOPY (1.98 Å)