7JZX

Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF7

7JZX の概要

• エントリーDOI: 10.2210/pdb7jzx/pdb
• EMDBエントリー: 22582 22583 22584 22585
• 分子名称: CRISPR-associated endonuclease Cas6/Csy4, Type I-F CRISPR-associated protein Csy2, CRISPR type I-F/YPEST-associated protein Csy3, ... (6 entities in total)
• 機能のキーワード: crispr, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 360931.75

構造登録者

Chang, L.,Li, Z.,Gabel, C. (登録日: 2020-09-02, 公開日: 2020-12-30, 最終更新日: 2025-05-14)

主引用文献

Gabel, C.,Li, Z.,Zhang, H.,Chang, L.
Structural basis for inhibition of the type I-F CRISPR-Cas surveillance complex by AcrIF4, AcrIF7 and AcrIF14.
Nucleic Acids Res., 49:584-594, 2021

PubMed Abstract: CRISPR-Cas systems are adaptive immune systems in bacteria and archaea to defend against mobile genetic elements (MGEs) and have been repurposed as genome editing tools. Anti-CRISPR (Acr) proteins are produced by MGEs to counteract CRISPR-Cas systems and can be used to regulate genome editing by CRISPR techniques. Here, we report the cryo-EM structures of three type I-F Acr proteins, AcrIF4, AcrIF7 and AcrIF14, bound to the type I-F CRISPR-Cas surveillance complex (the Csy complex) from Pseudomonas aeruginosa. AcrIF4 binds to an unprecedented site on the C-terminal helical bundle of Cas8f subunit, precluding conformational changes required for activation of the Csy complex. AcrIF7 mimics the PAM duplex of target DNA and is bound to the N-terminal DNA vise of Cas8f. Two copies of AcrIF14 bind to the thumb domains of Cas7.4f and Cas7.6f, preventing hybridization between target DNA and the crRNA. Our results reveal structural detail of three AcrIF proteins, each binding to a different site on the Csy complex for inhibiting degradation of MGEs.

PubMed: 33332569
DOI: 10.1093/nar/gkaa1199

実験手法

ELECTRON MICROSCOPY (3.4 Å)