7JYI

Subparticle Map of ZIKV MR-766

7JYI の概要

• エントリーDOI: 10.2210/pdb7jyi/pdb
• EMDBエントリー: 22526 22527
• 分子名称: E Glycoprotein, M protein (2 entities in total)
• 機能のキーワード: mr-766 zikv two-fold subparticle, viral protein
• 由来する生物種: Zika virus (ZIKV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125661.68

構造登録者

Jose, J.,Hafenstein, S. (登録日: 2020-08-30, 公開日: 2020-09-16, 最終更新日: 2025-05-28)

主引用文献

DiNunno, N.M.,Goetschius, D.J.,Narayanan, A.,Majowicz, S.A.,Moustafa, I.,Bator, C.M.,Hafenstein, S.L.,Jose, J.
Identification of a pocket factor that is critical to Zika virus assembly.
Nat Commun, 11:4953-4953, 2020

PubMed Abstract: Zika virus (ZIKV) is an emerging mosquito borne flavivirus and a major public health concern causing severe disease. Due to the presence of a lipid membrane and structural heterogeneity, attaining an atomic resolution structure is challenging, but important to understand virus assembly and life cycle mechanisms that offer distinct targets for therapeutic intervention. We here use subvolume refinement to achieve a 3.4 Å resolution structure and identify two distinct lipid moieties. The first arises from the inner leaflet and is coordinated by hydrophobic residues of the M and E transmembrane helices that form a binding pocket not previously characterized. The second lipid arises from the outer leaflet coordinate between two E protein helices. Structure-based mutagenesis identifies critical hydrophobic interactions and their effect on the virus life cycle. Results show that lipids play an essential role in the ZIKV assembly pathway revealing a potential target of lipid based antiviral drug development.

PubMed: 33009400
DOI: 10.1038/s41467-020-18747-4

実験手法

ELECTRON MICROSCOPY (3.4 Å)