7JXG

Structural model for Fe-containing human acireductone dioxygenase

7JXG の概要

• エントリーDOI: 10.2210/pdb7jxg/pdb
• NMR情報: BMRB: 50269
• 分子名称: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, FE (II) ION (3 entities in total)
• 機能のキーワード: cupin barrel, methionine salvage, cancer, oxidoreductase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21586.26

構造登録者

Pochapsky, T.C.,Liu, X.,Deshpande, A.,Ringe, D.,Garber, A.,Ryan, J. (登録日: 2020-08-27, 公開日: 2020-11-18, 最終更新日: 2024-05-01)

主引用文献

Liu, X.,Garber, A.,Ryan, J.,Deshpande, A.,Ringe, D.,Pochapsky, T.C.
A Model for the Solution Structure of Human Fe(II)-Bound Acireductone Dioxygenase and Interactions with the Regulatory Domain of Matrix Metalloproteinase I (MMP-I).
Biochemistry, 59:4238-4249, 2020

PubMed Abstract: The metalloenzyme acireductone dioxygenase (ARD) shows metal-dependent physical and enzymatic activities depending upon the metal bound in the active site. The Fe(II)-bound enzyme catalyzes the penultimate step of the methionine salvage pathway, converting 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one (acireductone) into formate and the ketoacid precursor of methionine, 2-keto-4-thiomethyl-2-oxobutanoate, using O as the oxidant. If Ni(II) is bound, an off-pathway shunt occurs, producing 3-methylthiopropionate, formate, and carbon monoxide from the same acireductone substrate. The solution structure of the Fe(II)-bound human enzyme, HsARD, is described and compared with the structures of Ni-bound forms of the closely related mouse enzyme, MmARD. Potential rationales for the different reactivities of the two isoforms are discussed. The human enzyme has been found to regulate the activity of matrix metalloproteinase I (MMP-I), which is involved in tumor metastasis, by binding the cytoplasmic transmembrane tail peptide of MMP-I. Nuclear magnetic resonance titration of HsARD with the MMP-I tail peptide permits identification of the peptide binding site on HsARD, a cleft anterior to the metal binding site adjacent to a dynamic proline-rich loop.

PubMed: 33135413
DOI: 10.1021/acs.biochem.0c00724

実験手法

SOLUTION NMR