7JXE

Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology

7JXE の概要

• エントリーDOI: 10.2210/pdb7jxe/pdb
• 分子名称: S2X35 antigen-binding (Fab) fragment (3 entities in total)
• 機能のキーワード: fusion protein, neutralizing antibody, sarbecovirus, structural genomics, seattle structural genomics center for infectious disease, ssgcid, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47725.07

構造登録者

Tortorici, M.A.,Park, Y.J.,Walls, A.C.,Czudnochowski, N.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),Snell, G.,Veesler, D. (登録日: 2020-08-27, 公開日: 2020-10-14, 最終更新日: 2024-10-23)

主引用文献

Piccoli, L.,Park, Y.J.,Tortorici, M.A.,Czudnochowski, N.,Walls, A.C.,Beltramello, M.,Silacci-Fregni, C.,Pinto, D.,Rosen, L.E.,Bowen, J.E.,Acton, O.J.,Jaconi, S.,Guarino, B.,Minola, A.,Zatta, F.,Sprugasci, N.,Bassi, J.,Peter, A.,De Marco, A.,Nix, J.C.,Mele, F.,Jovic, S.,Rodriguez, B.F.,Gupta, S.V.,Jin, F.,Piumatti, G.,Lo Presti, G.,Pellanda, A.F.,Biggiogero, M.,Tarkowski, M.,Pizzuto, M.S.,Cameroni, E.,Havenar-Daughton, C.,Smithey, M.,Hong, D.,Lepori, V.,Albanese, E.,Ceschi, A.,Bernasconi, E.,Elzi, L.,Ferrari, P.,Garzoni, C.,Riva, A.,Snell, G.,Sallusto, F.,Fink, K.,Virgin, H.W.,Lanzavecchia, A.,Corti, D.,Veesler, D.
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.
Cell, 183:1024-1042.e21, 2020

PubMed Abstract: Analysis of the specificity and kinetics of neutralizing antibodies (nAbs) elicited by SARS-CoV-2 infection is crucial for understanding immune protection and identifying targets for vaccine design. In a cohort of 647 SARS-CoV-2-infected subjects, we found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores. The receptor-binding domain (RBD) is immunodominant and the target of 90% of the neutralizing activity present in SARS-CoV-2 immune sera. Whereas overall RBD-specific serum IgG titers waned with a half-life of 49 days, nAb titers and avidity increased over time for some individuals, consistent with affinity maturation. We structurally defined an RBD antigenic map and serologically quantified serum Abs specific for distinct RBD epitopes leading to the identification of two major receptor-binding motif antigenic sites. Our results explain the immunodominance of the receptor-binding motif and will guide the design of COVID-19 vaccines and therapeutics.

PubMed: 32991844
DOI: 10.1016/j.cell.2020.09.037

実験手法

X-RAY DIFFRACTION (2.043 Å)