7JWR

Cellular retinol-binding protein 2 (CRBP2) in complex with 2-oleoylglycerol

7JWR の概要

• エントリーDOI: 10.2210/pdb7jwr/pdb
• 分子名称: Retinol-binding protein 2, 1,3-dihydroxypropan-2-yl (9Z)-octadec-9-enoate (3 entities in total)
• 機能のキーワード: oleoylglycerol, monoacylglycerol, retinol-binding protein, lipid binding, lipid binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33103.57

構造登録者

Silvaroli, J.A.,Banarjee, S.,Golczak, M. (登録日: 2020-08-26, 公開日: 2021-03-10, 最終更新日: 2023-10-18)

主引用文献

Silvaroli, J.A.,Plau, J.,Adams, C.H.,Banerjee, S.,Widjaja-Adhi, M.A.K.,Blaner, W.S.,Golczak, M.
Molecular basis for the interaction of cellular retinol binding protein 2 (CRBP2) with nonretinoid ligands.
J.Lipid Res., 62:100054-100054, 2021

PubMed Abstract: Present in the small intestine, cellular retinol binding protein 2 (CRBP2) plays an important role in the uptake, transport, and metabolism of dietary retinoids. However, the recent discovery of the interactions of CRBP2 with 2-arachidonoylglycerol and other monoacylglycerols (MAGs) suggests the broader involvement of this protein in lipid metabolism and signaling. To better understand the physiological role of CRBP2, we determined its protein-lipid interactome using a fluorescence-based retinol replacement assay adapted for a high-throughput screening format. By examining chemical libraries of bioactive lipids, we provided evidence for the selective interaction of CRBP2 with a subset of nonretinoid ligands with the highest affinity for sn-1 and sn-2 MAGs that contain polyunsaturated C18-C20 acyl chains. We also elucidated the structure-affinity relationship for nonretinoid ligands of this protein. We further dissect the molecular basis for this ligand's specificity by analyzing high-resolution crystal structures of CRBP2 in complex with selected derivatives of MAGs. Finally, we identify T51 and V62 as key amino acids that enable the broadening of ligand selectivity to MAGs in CRBP2 as compared with retinoid-specific CRBP1. Thus, our study provides the molecular framework for understanding the lipid selectivity and diverse functions of CRBPs in controlling lipid homeostasis.

PubMed: 33631211
DOI: 10.1016/j.jlr.2021.100054

実験手法

X-RAY DIFFRACTION (1.30000068656 Å)