7JTR

Complex of maltose-binding protein (MBP) with single-chain Fv (scFv)

7JTR の概要

• エントリーDOI: 10.2210/pdb7jtr/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900001
• 分子名称: Maltose/maltodextrin-binding periplasmic protein, single-chain Fv antibody fragment (scFv), alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: maltose, antibody, scfv, complex, sugar binding protein, sugar binding protein-immune system complex, sugar binding protein/immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 272447.75

構造登録者

Loll, P.J. (登録日: 2020-08-18, 公開日: 2021-05-05, 最終更新日: 2024-11-13)

主引用文献

Lenon, M.,Ke, N.,Ren, G.,Meuser, M.E.,Loll, P.J.,Riggs, P.,Berkmen, M.
A useful epitope tag derived from maltose binding protein.
Protein Sci., 30:1235-1246, 2021

PubMed Abstract: Maltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross-reactivity to other proteins in an Escherichia coli lysate. This high level of specificity suggested that MBP contains an epitope that could prove useful as a purification and visualization tag for proteins expressed in E. coli. To discover the MBP epitope, a co-crystal structure was determined for MBP bound to its antibody and four amino acids of MBP were identified as critical for the binding interaction. Fusions of various fragments of MBP to the glutathione S-transferase protein were engineered in order to identify the smallest fragment still recognized by the α-MBP antibody. Stabilization of the epitope via mutational engineering resulted in a minimized 14 amino-acid tag.

PubMed: 33896065
DOI: 10.1002/pro.4088

実験手法

X-RAY DIFFRACTION (2.5 Å)