7JSX

EPYC1(106-135) peptide-bound Rubisco

7JSX の概要

• エントリーDOI: 10.2210/pdb7jsx/pdb
• EMDBエントリー: 22308 22401 22462
• 分子名称: Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain 2, chloroplastic, EPYC1, ... (4 entities in total)
• 機能のキーワード: rubisco, pyrenoid, plant protein, lyase
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 612563.58

構造登録者

Matthies, D.,He, S.,Jonikas, M.C. (登録日: 2020-08-16, 公開日: 2020-11-18, 最終更新日: 2024-11-06)

主引用文献

He, S.,Chou, H.T.,Matthies, D.,Wunder, T.,Meyer, M.T.,Atkinson, N.,Martinez-Sanchez, A.,Jeffrey, P.D.,Port, S.A.,Patena, W.,He, G.,Chen, V.K.,Hughson, F.M.,McCormick, A.J.,Mueller-Cajar, O.,Engel, B.D.,Yu, Z.,Jonikas, M.C.
The structural basis of Rubisco phase separation in the pyrenoid.
Nat.Plants, 6:1480-1490, 2020

PubMed Abstract: Approximately one-third of global CO fixation occurs in a phase-separated algal organelle called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase separation of the CO-fixing enzyme Rubisco with a linker protein; however, the molecular interactions underlying this phase separation remain unknown. Here we present the structural basis of the interactions between Rubisco and its intrinsically disordered linker protein Essential Pyrenoid Component 1 (EPYC1) in the model alga Chlamydomonas reinhardtii. We find that EPYC1 consists of five evenly spaced Rubisco-binding regions that share sequence similarity. Single-particle cryo-electron microscopy of these regions in complex with Rubisco indicates that each Rubisco holoenzyme has eight binding sites for EPYC1, one on each Rubisco small subunit. Interface mutations disrupt binding, phase separation and pyrenoid formation. Cryo-electron tomography supports a model in which EPYC1 and Rubisco form a codependent multivalent network of specific low-affinity bonds, giving the matrix liquid-like properties. Our results advance the structural and functional understanding of the phase separation underlying the pyrenoid, an organelle that plays a fundamental role in the global carbon cycle.

PubMed: 33230314
DOI: 10.1038/s41477-020-00811-y

実験手法

ELECTRON MICROSCOPY (2.06 Å)