7JRR

Crystal structures of artificially designed homomeric RNA nanoarchitectures

7JRR の概要

• エントリーDOI: 10.2210/pdb7jrr/pdb
• 分子名称: RNA (50-MER), MAGNESIUM ION, MANGANESE (II) ION, ... (4 entities in total)
• 機能のキーワード: nano structure, dimeric parallelogram, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17073.12

構造登録者

Liu, D.,Shao, Y.,Piccirilli, J.A.,Weizmann, Y. (登録日: 2020-08-12, 公開日: 2021-09-08, 最終更新日: 2023-10-18)

主引用文献

Liu, D.,Shao, Y.,Piccirilli, J.A.,Weizmann, Y.
Structures of artificially designed discrete RNA nanoarchitectures at near-atomic resolution.
Sci Adv, 7:eabf4459-eabf4459, 2021

PubMed Abstract: Although advances in nanotechnology have enabled the construction of complex and functional synthetic nucleic acid–based nanoarchitectures, high-resolution discrete structures are lacking because of the difficulty in obtaining good diffracting crystals. Here, we report the design and construction of RNA nanostructures based on homooligomerizable one-stranded tiles for x-ray crystallographic determination. We solved three structures to near-atomic resolution: a 2D parallelogram, a 3D nanobracelet unexpectedly formed from an RNA designed for a nanocage, and, eventually, a bona fide 3D nanocage designed with the guidance of the two previous structures. Structural details of their constituent motifs, such as kissing loops, branched kissing loops, and T-junctions, that resemble natural RNA motifs and resisted x-ray determination are revealed, providing insights into those natural motifs. This work unveils the largely unexplored potential of crystallography in gaining high-resolution feedback for nanoarchitectural design and suggests a route to investigate RNA motif structures by configuring them into nanoarchitectures.

PubMed: 34550747
DOI: 10.1126/sciadv.abf4459

実験手法

X-RAY DIFFRACTION (2.16 Å)