7JR9

Chlamydomonas reinhardtii radial spoke minimal head complex

7JR9 の概要

• エントリーDOI: 10.2210/pdb7jr9/pdb
• EMDBエントリー: 22444
• 分子名称: Radial spoke protein 9, Flagellar radial spoke protein 4, Flagellar radial spoke protein 6, ... (6 entities in total)
• 機能のキーワード: cilia, radial spoke, mechano-regulation, structural protein
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 184969.88

構造登録者

Grossman-Haham, I.,Coudray, N.,Yu, Z.,Wang, F.,Bhabha, G.,Vale, R.D. (登録日: 2020-08-11, 公開日: 2020-12-16, 最終更新日: 2024-11-06)

主引用文献

Grossman-Haham, I.,Coudray, N.,Yu, Z.,Wang, F.,Zhang, N.,Bhabha, G.,Vale, R.D.
Structure of the radial spoke head and insights into its role in mechanoregulation of ciliary beating.
Nat.Struct.Mol.Biol., 28:20-28, 2021

PubMed Abstract: Motile cilia power cell locomotion and drive extracellular fluid flow by propagating bending waves from their base to tip. The coordinated bending of cilia requires mechanoregulation by the radial spoke (RS) protein complexes and the microtubule central pair (CP). Despite their importance for ciliary motility across eukaryotes, the molecular function of the RSs is unknown. Here, we reconstituted the Chlamydomonas reinhardtii RS head that abuts the CP and determined its structure using single-particle cryo-EM to 3.1-Å resolution, revealing a flat, negatively charged surface supported by a rigid core of tightly intertwined proteins. Mutations in this core, corresponding to those involved in human ciliopathies, compromised the stability of the recombinant complex, providing a molecular basis for disease. Partially reversing the negative charge on the RS surface impaired motility in C. reinhardtii. We propose that the RS-head architecture is well-suited for mechanoregulation of ciliary beating through physical collisions with the CP.

PubMed: 33318704
DOI: 10.1038/s41594-020-00519-9

実験手法

ELECTRON MICROSCOPY (2.95 Å)