7JR5

Real Time Reaction Intermediates in Stigmatella Bacteriophytochrome P2

7JR5 の概要

• エントリーDOI: 10.2210/pdb7jr5/pdb
• 分子名称: Photoreceptor-histidine kinase BphP, 3-[2-[[5-[(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-5-[[(3~{S})-4-ethyl-3-methyl-2-oxidanylidene-1,3-dihydropyrrol-5-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid, BENZAMIDINE, ... (4 entities in total)
• 機能のキーワード: phytochrome, photosensory core domain, time-resolved serial femtosecond crystallography, nanosecond, red/far-red, z to e isomerization, signaling protein
• 由来する生物種: Stigmatella aurantiaca (strain DW4/3-1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106942.52

構造登録者

Schmidt, M. (登録日: 2020-08-11, 公開日: 2021-10-06, 最終更新日: 2024-10-16)

主引用文献

Carrillo, M.,Pandey, S.,Sanchez, J.,Noda, M.,Poudyal, I.,Aldama, L.,Malla, T.N.,Claesson, E.,Wahlgren, W.Y.,Feliz, D.,Srajer, V.,Maj, M.,Castillon, L.,Iwata, S.,Nango, E.,Tanaka, R.,Tanaka, T.,Fangjia, L.,Tono, K.,Owada, S.,Westenhoff, S.,Stojkovic, E.A.,Schmidt, M.
High-resolution crystal structures of transient intermediates in the phytochrome photocycle.
Structure, 29:743-754.e4, 2021

PubMed Abstract: Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display a reversible photocycle between the resting Pr state and the light-activated Pfr state. Light signals are transduced as structural change through the entire protein to modulate its activity. It is unknown how the Pr-to-Pfr interconversion occurs, as the structure of intermediates remains notoriously elusive. Here, we present short-lived crystal structures of the photosensory core modules of the bacteriophytochrome from myxobacterium Stigmatella aurantiaca captured by an X-ray free electron laser 5 ns and 33 ms after light illumination of the Pr state. We observe large structural displacements of the covalently bound bilin chromophore, which trigger a bifurcated signaling pathway that extends through the entire protein. The snapshots show with atomic precision how the signal progresses from the chromophore, explaining how plants, bacteria, and fungi sense red light.

PubMed: 33756101
DOI: 10.1016/j.str.2021.03.004

実験手法

X-RAY DIFFRACTION (2.4 Å)