7JPU

Structure of an endocytic receptor

7JPU の概要

• エントリーDOI: 10.2210/pdb7jpu/pdb
• EMDBエントリー: 22423
• 分子名称: Lymphocyte antigen 75 (1 entity in total)
• 機能のキーワード: cell-surface receptor, immune receptor, mannose receptor family, immune system
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 794212.69

構造登録者

Gully, B.S.,Rossjohn, J.,Berry, R. (登録日: 2020-08-09, 公開日: 2020-12-09, 最終更新日: 2024-11-06)

主引用文献

Gully, B.S.,Venugopal, H.,Fulcher, A.J.,Fu, Z.,Li, J.,Deuss, F.A.,Llerena, C.,Heath, W.R.,Lahoud, M.H.,Caminschi, I.,Rossjohn, J.,Berry, R.
The cryo-EM structure of the endocytic receptor DEC-205.
J.Biol.Chem., 296:100127-100127, 2020

PubMed Abstract: DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine oligonucleotides, a motif often seen in bacterial or viral DNA. However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. Here, we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. The DEC-205 monomer forms a compact structure comprising two intercalated rings of C-type lectin-like domains, where the N-terminal cysteine-rich and fibronectin domains reside at the central intersection. We establish a pH-dependent oligomerization pathway forming tetrameric DEC-205 using solution-based techniques and ultimately solved the 4.9 Å cryo-EM structure of the DEC-205 tetramer to identify the unfurling of the second lectin ring which enables tetramer formation. Furthermore, we suggest the relevance of this oligomerization pathway within a cellular setting, whereby cytosine-guanosine binding appeared to disrupt this cell-surface oligomer. Accordingly, we provide insight into the structure and oligomeric assembly of the DEC-205 receptor.

PubMed: 33257321
DOI: 10.1074/jbc.RA120.016451

実験手法

ELECTRON MICROSCOPY (5 Å)