7JMU

Hen egg-white lysozyme with ionic liquid ethylammonium nitrate

7JMU の概要

• エントリーDOI: 10.2210/pdb7jmu/pdb
• 分子名称: Lysozyme C, NITRATE ION (3 entities in total)
• 機能のキーワード: lysozyme, ionic liquid, nitrate, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29592.40

構造登録者

Han, Q.,Darmanin, C.,Smith, K.,Greaves, T.,Drummond, C. (登録日: 2020-08-02, 公開日: 2020-11-11, 最終更新日: 2024-10-30)

主引用文献

Han, Q.,Smith, K.M.,Darmanin, C.,Ryan, T.M.,Drummond, C.J.,Greaves, T.L.
Lysozyme conformational changes with ionic liquids: Spectroscopic, small angle x-ray scattering and crystallographic study.
J Colloid Interface Sci, 585:433-443, 2021

PubMed Abstract: Solvents that support protein functionality are important for biochemical applications, and new solvents are required. Here we employ FTIR and fluorescence spectroscopies, small angle X-ray scattering (SAXS) and X-ray crystallography to understand conformational changes of lysozyme with ionic liquids (ILs) added. Spectroscopic techniques identified that the secondary structure of lysozyme was maintained at the lower IL concentrations of 1 and 5 mol%, though the Tryptophan environment was significantly altered with nitrate-based ILs present. SAXS experiments indicated that the radius of gyration of lysozyme increased with 1 mol% IL present, and then decreased with increasing IL concentrations. The tertiary structure, particularly the loop regions, changed as a function of IL concentration, and this depended on the IL type. The crystallographic structure of lysozyme with the IL of ethylammonium nitrate present confirmed the loop region was extended, and identified three specific binding sites with nitrate ions, and that the positively charged areas were IL sensitive regions. This work provides a detailed understanding of lysozyme conformational changes in the presence of ILs. This approach can be extended to other functionally-important proteins.

PubMed: 33109332
DOI: 10.1016/j.jcis.2020.10.024

実験手法

X-RAY DIFFRACTION (1.2 Å)