7JLU

Structure of the activated Roq1 resistosome directly recognizing the pathogen effector XopQ

7JLU の概要

• エントリーDOI: 10.2210/pdb7jlu/pdb
• EMDBエントリー: 22380
• 分子名称: Disease resistance protein Roq1, XopQ, CALCIUM ION (3 entities in total)
• 機能のキーワード: resistosome, plant immunity, effector, lrr, tir, nb-arc, pl., immune system
• 由来する生物種: Nicotiana benthamiana; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 204748.68

構造登録者

Martin, R.,Qi, T.,Zhang, H.,Lui, F.,King, M.,Toth, C.,Nogales, E.,Staskawicz, B.J. (登録日: 2020-07-30, 公開日: 2020-12-02, 最終更新日: 2024-03-06)

主引用文献

Martin, R.,Qi, T.,Zhang, H.,Liu, F.,King, M.,Toth, C.,Nogales, E.,Staskawicz, B.J.
Structure of the activated ROQ1 resistosome directly recognizing the pathogen effector XopQ.
Science, 370:-, 2020

PubMed Abstract: Plants and animals detect pathogen infection using intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) that directly or indirectly recognize pathogen effectors and activate an immune response. How effector sensing triggers NLR activation remains poorly understood. Here we describe the 3.8-angstrom-resolution cryo-electron microscopy structure of the activated ROQ1 (recognition of XopQ 1), an NLR native to with a Toll-like interleukin-1 receptor (TIR) domain bound to the effector XopQ ( outer protein Q). ROQ1 directly binds to both the predicted active site and surface residues of XopQ while forming a tetrameric resistosome that brings together the TIR domains for downstream immune signaling. Our results suggest a mechanism for the direct recognition of effectors by NLRs leading to the oligomerization-dependent activation of a plant resistosome and signaling by the TIR domain.

PubMed: 33273074
DOI: 10.1126/science.abd9993

実験手法

ELECTRON MICROSCOPY (3.8 Å)