7JIL の概要
| エントリーDOI | 10.2210/pdb7jil/pdb |
| EMDBエントリー | 22345 |
| 分子名称 | 50S ribosomal protein L2, 50S ribosomal protein L16, 50S ribosomal protein L17, ... (54 entities in total) |
| 機能のキーワード | translation initiation, protein synthesis, bs21, ribosome |
| 由来する生物種 | Flavobacterium johnsoniae 詳細 |
| タンパク質・核酸の鎖数 | 53 |
| 化学式量合計 | 2137364.67 |
| 構造登録者 | |
| 主引用文献 | Jha, V.,Roy, B.,Jahagirdar, D.,McNutt, Z.A.,Shatoff, E.A.,Boleratz, B.L.,Watkins, D.E.,Bundschuh, R.,Basu, K.,Ortega, J.,Fredrick, K. Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome. Nucleic Acids Res., 49:547-567, 2021 Cited by PubMed Abstract: Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the Bacteroidetes, fail to recognize the SD sequence of mRNA in vitro. A cryo-electron microscopy structure of the complete 70S ribosome from F. johnsoniae at 2.8 Å resolution reveals that the ASD is sequestered by ribosomal proteins bS21, bS18 and bS6, explaining the basis of ASD inhibition. The structure also uncovers a novel ribosomal protein-bL38. Remarkably, in F. johnsoniae and many other Flavobacteriia, the gene encoding bS21 contains a strong SD, unlike virtually all other genes. A subset of Flavobacteriia have an alternative ASD, and in these organisms the fully complementary sequence lies upstream of the bS21 gene, indicative of natural covariation. In other Bacteroidetes classes, strong SDs are frequently found upstream of the genes for bS21 and/or bS18. We propose that these SDs are used as regulatory elements, enabling bS21 and bS18 to translationally control their own production. PubMed: 33330920DOI: 10.1093/nar/gkaa1195 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (2.8 Å) |
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