7JH1

Solution structure of a reconstructed XCL1 ancestor

7JH1 の概要

• エントリーDOI: 10.2210/pdb7jh1/pdb
• NMR情報: BMRB: 30777
• 分子名称: XCL1 ancestor (1 entity in total)
• 機能のキーワード: metamorphic protein, chemokine, ancestral reconstruction, cytokine
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7483.08

構造登録者

Tyler, R.C.,Peterson, F.C.,Volkman, B.F. (登録日: 2020-07-20, 公開日: 2020-12-30, 最終更新日: 2024-10-30)

主引用文献

Dishman, A.F.,Tyler, R.C.,Fox, J.C.,Kleist, A.B.,Prehoda, K.E.,Babu, M.M.,Peterson, F.C.,Volkman, B.F.
Evolution of fold switching in a metamorphic protein.
Science, 371:86-90, 2021

PubMed Abstract: Metamorphic proteins switch between different folds, defying the protein folding paradigm. It is unclear how fold switching arises during evolution. With ancestral reconstruction and nuclear magnetic resonance, we studied the evolution of the metamorphic human protein XCL1, which has two distinct folds with different functions, making it an unusual member of the chemokine family, whose members generally adopt one conserved fold. XCL1 evolved from an ancestor with the chemokine fold. Evolution of a dimer interface, changes in structural constraints and molecular strain, and alteration of intramolecular protein contacts drove the evolution of metamorphosis. Then, XCL1 likely evolved to preferentially populate the noncanonical fold before reaching its modern-day near-equal population of folds. These discoveries illuminate how one sequence has evolved to encode multiple structures, revealing principles for protein design and engineering.

PubMed: 33384377
DOI: 10.1126/science.abd8700

実験手法

SOLUTION NMR