7JGY

Solution NMR structure of protonectin, a peptide from wasp

7JGY の概要

• エントリーDOI: 10.2210/pdb7jgy/pdb
• NMR情報: BMRB: 30776
• 分子名称: Protonectin peptide ILE-LEU-GLY-THR-ILE-LEU-GLY-LEU-LEU-LYS-GLY-LEU-NH2 (1 entity in total)
• 機能のキーワード: alpha-helical, antimicrobial, non-haemolytic, antimicrobial protein
• 由来する生物種: Parachartergus fraternus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1209.57

構造登録者

Muller, J.A.I.,Craik, D.J.,Koehbach, J. (登録日: 2020-07-20, 公開日: 2020-12-16, 最終更新日: 2023-06-14)

主引用文献

Muller, J.A.I.,Lawrence, N.,Chan, L.Y.,Harvey, P.J.,Elliott, A.G.,Blaskovich, M.A.T.,Goncalves, J.C.,Galante, P.,Mortari, M.R.,Toffoli-Kadri, M.C.,Koehbach, J.,Craik, D.J.
Antimicrobial and Anticancer Properties of Synthetic Peptides Derived from the Wasp Parachartergus fraternus.
Chembiochem, 22:1415-1423, 2021

PubMed Abstract: Agelaia-MPI and protonectin are antimicrobial peptides isolated from the wasp Parachartergus fraternus that show antimicrobial and neuroactive activities. Previously, two analogues of these peptides, neuroVAL and protonectin-F, were designed to reduce nonspecific toxicity and improve potency. Here, the three-dimensional structures of neuroVAL, protonectin and protonectin-F were determined by using circular dichroism and NMR spectroscopy. Antibacterial, antifungal, cytotoxic and hemolytic activities were tested for the parent peptides and analogues. All peptides showed moderate antimicrobial activity against Gram-positive bacteria, with agelaia-MPI being the most active. Protonectin and protonectin-F were found to be toxic to cancerous and noncancerous cell lines. Internalization experiments revealed that these peptides accumulate inside both cell types. By contrast, neuroVAL was nontoxic to all tested cells and was able to enter cells without accumulating. In summary, neuroVAL has potential as a nontoxic cell-penetrating peptide, while protonectin-F needs further modification to realize its potential as an antitumor peptide.

PubMed: 33244888
DOI: 10.1002/cbic.202000716

実験手法

SOLUTION NMR