7JGR

Structure of Drosophila ORC bound to DNA (84 bp) and Cdc6

7JGR の概要

• エントリーDOI: 10.2210/pdb7jgr/pdb
• 関連するPDBエントリー: 7JGS 7JK2 7JK3 7JK4 7JK5 7JK6
• EMDBエントリー: 22329 22330 22359 22360 22361 22362 22363
• 分子名称: Origin recognition complex subunit 2, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (11 entities in total)
• 機能のキーワード: origin recognition complex, dna replication initiation, dna binding, replication
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 441646.64

構造登録者

Schmidt, J.M.,Bleichert, F. (登録日: 2020-07-19, 公開日: 2020-09-09, 最終更新日: 2024-03-06)

主引用文献

Schmidt, J.M.,Bleichert, F.
Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6.
Nat Commun, 11:4263-4263, 2020

PubMed Abstract: Eukaryotic DNA replication initiation relies on the origin recognition complex (ORC), a DNA-binding ATPase that loads the Mcm2-7 replicative helicase onto replication origins. Here, we report cryo-electron microscopy (cryo-EM) structures of DNA-bound Drosophila ORC with and without the co-loader Cdc6. These structures reveal that Orc1 and Orc4 constitute the primary DNA binding site in the ORC ring and cooperate with the winged-helix domains to stabilize DNA bending. A loop region near the catalytic Walker B motif of Orc1 directly contacts DNA, allosterically coupling DNA binding to ORC's ATPase site. Correlating structural and biochemical data show that DNA sequence modulates DNA binding and remodeling by ORC, and that DNA bending promotes Mcm2-7 loading in vitro. Together, these findings explain the distinct DNA sequence-dependencies of metazoan and S. cerevisiae initiators in origin recognition and support a model in which DNA geometry and bendability contribute to Mcm2-7 loading site selection in metazoans.

PubMed: 32848132
DOI: 10.1038/s41467-020-18067-7

実験手法

ELECTRON MICROSCOPY (3.9 Å)