7INS

STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z

7INS の概要

• エントリーDOI: 10.2210/pdb7ins/pdb
• 分子名称: INSULIN (CHAIN A), INSULIN (CHAIN B), GENERAL PROTAMINE CHAIN, ... (7 entities in total)
• 機能のキーワード: hormone
• 由来する生物種: Sus scrofa (pig); 詳細
• 細胞内の位置: Secreted: P01315 P01315
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 19338.63

構造登録者

Balschmidt, P.,Hansen, F.B.,Dodson, E.,Dodson, G.,Korber, F. (登録日: 1991-09-03, 公開日: 1994-01-31, 最終更新日: 2025-02-12)

主引用文献

Balschmidt, P.,Hansen, F.B.,Dodson, E.J.,Dodson, G.G.,Korber, F.
Structure of porcine insulin cocrystallized with clupeine Z.
Acta Crystallogr.,Sect.B, 47:975-986, 1991

PubMed Abstract: The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods. The final crystallographic R factor for all reflections between 2 and 10 A is 19.4%. The insulin molecules are arranged as hexamers with two tetrahedrally coordinated Zn atoms in the central channel and one m-cresol bound to each monomer near His B5. One protamine binding site has been unequivocally identified near a dimer-dimer interface, although most of the polypeptide is crystallographically disordered. The conformation of the insulin moiety and the structural differences between the three unique monomers have been analysed. The zinc and m-cresol environments are described and the nature of the protamine binding site is outlined.

PubMed: 1772633
DOI: 10.1107/S010876819100842X

実験手法

X-RAY DIFFRACTION (2 Å)