7GSP

RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE

7GSP の概要

• エントリーDOI: 10.2210/pdb7gsp/pdb
• 分子名称: RIBONUCLEASE T1, PHOSPHATE ION, GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE, ... (4 entities in total)
• 機能のキーワード: hydrolase, endoribonuclease, ribonuclease, endonuclease
• 由来する生物種: Aspergillus oryzae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23101.87

構造登録者

Zegers, I.,Wyns, L. (登録日: 1997-12-10, 公開日: 1998-03-18, 最終更新日: 2023-08-09)

主引用文献

Zegers, I.,Loris, R.,Dehollander, G.,Fattah Haikal, A.,Poortmans, F.,Steyaert, J.,Wyns, L.
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.
Nat.Struct.Biol., 5:280-283, 1998

PubMed Abstract: Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.

PubMed: 9546218
DOI: 10.1038/nsb0498-280

実験手法

X-RAY DIFFRACTION (2 Å)